Ultrasound imaging for measuring muscle and subcutaneous fat tissue thickness of the anterior thigh: A two-year longitudinal study in middle age

Filippo Mechelli

Abstract


Background Ultrasound (US) imaging technique is widely used in research and clinical settings to assess the morphology and morphometry of neuromusculoskeletal structures. The technique has reported validity and reliability in measuring the size of various muscles under controlled conditions. The aim of the present study was to assess anterior thigh thickness using US imaging, in a healthy cohort of middle-aged older adults.

Methods Participants included seventeen healthy older adults involved in regular moderate-vigorous activities (age range 39-66 years). US imaging scans of the anterior thighs two years since baseline measurements were performed. Images were analyzed off-line to compare US imaging measurements of muscle thickness and subcutaneous fat (SF) of the anterior thigh taken at baseline and after two years.

Results There was no significant difference between muscle thickness measurements taken at baseline and after two years (Mean, standard deviation; baseline=2.80±0.71cm; follow-up=2.77±0.72cm, p=0.33). There was also no significant change in SF thickness (baseline=1.04±0.41cm; follow-up=1.06±0.40, p=0.33).

Conclusions The results show there was no decline in anterior thigh muscle thickness or increase in SF in the healthy cohort studied using US imaging over a two-year period. These findings demonstrate the robustness of US imaging measurements over time.


References


Whittaker JL, Teyhen DS, Elliott JM, Cook K, Langevin HM, Dahl HH et al. Rehabilitative Ultrasound Imaging: Understanding the Technology and Its Applications. J Orthop Sport Phys 2007;37:434–449.

Lieber RL. Skeletal muscle structure and function: implications for rehabilitation and sports medicine. Williams & Wilkins: Baltimore; 1992.

Wakefield RJ, Balint PV, Szkudlarek M, Filippucci E, Backhaus M, D’Agostino M-A et al. Musculoskeletal ultrasound including definitions for ultrasonographic pathology. J Rheumatol 2005;32:2485–2487.

Agyapong-Badu S, Warner M, Samuel D, Narici M, Cooper C, Stokes M. Anterior thigh composition measured using ultrasound imaging to quantify relative thickness of muscle and non-contractile tissue: a potential biomarker for musculoskeletal health. Physiol. Meas 2014;35:2165–2176.

Mechelli F, Arendt-Nielsen L, Stokes M, Agyapong-Badu S. Inter-rater and intra-rater reliability of ultrasound imaging for measuring quadriceps muscle and non-contractile tissue thickness of the anterior thigh. Biomed Phys Eng Express 2019;5:037002.

Whittaker JL, Emery CA. Sonographic Measures of the Gluteus Medius, Gluteus Minimus, and Vastus Medialis Muscles. J Orthop Sport Phys 2014;44:627–632.

Worsley PR, Kitsell F, Samuel D, Stokes M. Validity of measuring distal vastus medialis muscle using rehabilitative ultrasound imaging versus magnetic resonance imaging. Man Ther 2014;19:259–263.

Mechelli F, Arendt-Nielsen L, Stokes M, Agyapong-Badu S. Validity of Ultrasound Imaging Versus Magnetic Resonance Imaging for Measuring Anterior Thigh Muscle, Subcutaneous Fat, and Fascia Thickness. Methods Protoc 2019;2:58.

Mendis MD, Wilson SJ, Stanton W, Hides JA. Validity of Real-Time Ultrasound Imaging to Measure Anterior Hip Muscle Size: A Comparison With Magnetic Resonance Imaging. J Orthop Sport Phys 2010;40:577–581.

Petterson SC, Barrance P, Buchanan T, Binder-Macleod S, Snyder-Mackler L. Mechanisms Underlying Quadriceps Weakness in Knee Osteoarthritis: Med. Sci. Sports Exerc. 2008;40:422–427.

Henriksen M, Rosager S, Aaboe J, Graven-Nielsen T, Bliddal H. Experimental Knee Pain Reduces Muscle Strength. J. Pain 2011;12:460–467.

Rice DA, McNair PJ, Lewis GN, Dalbeth N. Quadriceps arthrogenic muscle inhibition: the effects of experimental knee joint effusion on motor cortex excitability. Arthritis Res Ther 2014;16.

Guleria R, Mohan A, Madan K, Mittal S, Kumar R, Hadda V et al. Intra- and Inter-Observer Reliability of Quadriceps Muscle Thickness Measured with Bedside Ultrasonography by Critical Care Physicians. Indian J. Crit. Care Med 2017;21:448–452.

Galindo Martín CA, Monares Zepeda E, Lescas Méndez OA. Bedside Ultrasound Measurement of Rectus Femoris: A Tutorial for the Nutrition Support Clinician. J Clin Nutr Metab 2017;2017:1–5.

Toledo DO, Silva DC de L e, Santos DM dos, Freitas BJ de, Dib R, Cordioli RL et al. Bedside ultrasound is a practical measurement tool for assessing muscle mass. Rev Bras Ter Intensiva 2017;29.

Tillquist M, Kutsogiannis DJ, Wischmeyer PE, Kummerlen C, Leung R, Stollery D et al. Bedside Ultrasound Is a Practical and Reliable Measurement Tool for Assessing Quadriceps Muscle Layer Thickness. J Parenter Enteral Nutr 2014;38:886–890.

Puthucheary ZA, Rawal J, McPhail M, Connolly B, Ratnayake G, Chan P et al. Acute Skeletal Muscle Wasting in Critical Illness. JAMA 2013;310:1591.

Rosenberg IH. Sarcopenia: Origins and Clinical Relevance. J Nutr 1997;127:990S-991S.

Aversa Z, Costelli P, Muscaritoli M. Cancer-induced muscle wasting: latest findings in prevention and treatment. Ther Adv Med Oncol 2017;9:369–382.

Janssen I, Heymsfield SB, Wang Z, Ross R. Skeletal muscle mass and distribution in 468 men and women aged 18–88 yr. J Appl Physiol 2000;89:81–88.

Narici MV, Maffulli N. Sarcopenia: characteristics, mechanisms and functional significance. Br. Med. Bull 2010;95:139–159.

Physical Activity Guidelines Advisory Committee. Physical activity guidelines advisory committee scientific report. Part C-7. Washington, DC: U.S. Department of Health and Human Services 2018 https://health.gov/paguidelines/second-edition/report/pdf/pag_advisory_committee_report.pdf.

Delaney S, Worsley P, Warner M, Taylor M, Stokes M. Assessing contractile ability of the quadriceps muscle using ultrasound imaging. Muscle Nerve 2010;42:530–538.

Cruz-Jentoft AJ, Baeyens JP, Bauer JM, Boirie Y, Cederholm T, Landi F et al. Sarcopenia: European consensus on definition and diagnosis: Report of the European Working Group on Sarcopenia in Older People. Age Ageing 2010;39:412–423.

Loenneke JP, Thiebaud RS, Abe T. Estimating Site-Specific Muscle Loss: A Valuable Tool for Early Sarcopenia Detection? Rejuvenation Res 2014;17:496–498.

Valla FV, Young DK, Rabilloud M, Periasami U, John M, Baudin F et al. Thigh Ultrasound Monitoring Identifies Decreases in Quadriceps Femoris Thickness as a Frequent Observation in Critically Ill Children*: Pediatr Crit Care Med 2017;18:e339–e347.

Looijaard WGPM, Molinger J, Weijs PJM. Measuring and monitoring lean body mass in critical illness: Curr Opin Crit Care 2018;24:241–247.

Narici MV, de Boer MD. Disuse of the musculo-skeletal system in space and on earth. Eur J Appl Physiol 2011;111:403–420.

Reardon K, Galea M, Dennett X, Choong P, Byrne E. Quadriceps muscle wasting persists 5 months after total hip arthroplasty for osteoarthritis of the hip: a pilot study Intern Med J 2001;31:7–14.

Harris-Love MO, Seamon BA, Teixeira C, Ismail C. Ultrasound estimates of muscle quality in older adults: reliability and comparison of Photoshop and ImageJ for the grayscale analysis of muscle echogenicity. PeerJ 2016;4:e1721.

Laviola M, Priori R, D’Angelo MG, Aliverti A. Assessment of diaphragmatic thickness by ultrasonography in Duchenne muscular dystrophy (DMD) patients. PLoS One 2018;13:e0200582.




DOI: http://dx.doi.org/10.17987/jcsm-cr.v4i2.94

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